cGMP HTS Immunoassay Kit (Chemiluminescent)
Description:
cGMP HTS Immunoassay Kit (Chemiluminescent)
Trade Name:
Upstate (Millipore)
Qty/Pk:
192 assays
Product Overview:
Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Rabbit antibody, cGMP standard, and an Alkaline Phosphate chemiluminescent substrate. This kit contains antibody coated plates and all reagents required to perform 192 assays.
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Applications:
The cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive & rapid chemiluminescent quantitation of cyclic GMP from extracts, lysates or supernatants of cells of any species.
Key Applications:
ELISA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Brand Family:
Upstate
Sensitivity:
Cross-Reactivity
cGMP 100%,
cAMP <0.1%,
GTP <0.01%,
GMP <0.01%,
CTP <0.01%,
AMP <0.01%,
ADP <0.01%,
ATP <0.01%.
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Storage Conditions:
1 year at 4°C from date of shipment
Product Name:
cGMP HTS Immunoassay Kit (Chemiluminescent)
Alternate Names:
cyclic GMP ELISA
Materials Required but Not Delivered:
1. Multi-channel or repeating pipettes
2. Pipettors & tips capable of accurately measuring 10-1000 μL
3. Plate shaker (optional)
4. Graduated serological pipettes
5. Luminometer capable of reading 96-well microplates.
6. Polystyrene test tubes for standard and sample dilutions
7. Mechanical vortex
8. Containers for dilutions
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Analytes Available:
cGMP
Quality Assurance:
Routinely evaluated by ELISA.
Components:
1. White 96-Well anti-Rabbit Coated Plate: (Part No. 2004070) Two 96-well immuno-plates pre-coated with anti-Rabbit polyclonal antibody, sealed in a foil pouch.
2. Rabbit anti-cGMP polyclonal Antibody: (Part No. 2004761) One 25 μL vial.
3. cGMP Standard: (Part No. 2004762) One 2 mL bottle
4. Alkaline Phosphatase cGMP Tracer Conjugate: (Part No. 2004760) One 20 μL vial
5. 2x cGMP Assay Diluent: (Part No. 2004811) One 25 mL bottle.
6. 5x Wash Buffer: (Part No. 2004075) One 60 mL bottle.
7. Lysis Buffer: (Part No. 2004076) One 125 mL bottle.
8. 6x Alkaline Phosphatase Substrate: (Part No. 2004077) One 5 mL bottle.
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Detection Methods:
Chemiluminescence
Chemiluminescence |