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PTEN MALACHITE GREEN ASSAYKIT(-80C)

产品编号: 17-351DFR     查看说明书
产品名称: PTEN MALACHITE GREEN ASSAYKIT(-80C)  .0   订购此产品 
供应商: Millipore
规格: EA
目录价:
库存状态: 三周到货
CAS编号:
应用范围: 生化实验
种属来源:
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PTEN Malachite Green Assay Kit
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PRODUCT FAMILY INFORMATION
PTEN Antibodies
Millipore offers PTEN (phosphatase and tensin homolog deleted on chromosome ten) Antibodies, Kits, Proteins, and Milliplex products. See below for related products for PTEN.
Millipore’s Anti- PTEN Antibodies demonstrates specificity against PTEN. See below for data, references and related products for PTEN. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Description:
PTEN Malachite Green Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
This kit is based on quantitation of the green complex formed between Malachite Green, molybdate and free phosphate.
Applications:
This PTEN Malachite Green Assay Kit is based on quantitation of the green complex formed between Malachite Green, molybdate & free phosphate.
Key Applications:
Phosphatase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.
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UniProt Summary:
FUNCTION: SwissProt: P60484 # Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.
COFACTOR: Magnesium.
SIZE: 403 amino acids; 47166 Da
SUBUNIT: Monomer. The unphosphorylated form interacts with the second PDZ domain of AIP1 and with DLG1 and MAST2 in vitro.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
DOMAIN: SwissProt: P60484 The C2 domain binds phospholipid membranes in vitro in a Ca(2+)-independent manner; this binding is important for its tumor suppressor function.
PTM: Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both in

保存条件: RT
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