|
Anti-Citrulline (Modified) Detection Kit
Description:
Anti-Citrulline (Modified) Detection Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine in a strong acid solution to form a ureido group adduct. This ensures detection of citrulline-containing proteins regardless of neighboring amino acid sequences. Detection of the modified citrulline proteins is subsequently performed using a standard immunoblot protocol with the anti-Citrulline and goat anti-rabbit IgG-HRP.
View All »
Applications:
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody.
Key Applications:
Western Blotting
Immunohistochemistry
Species Reactivity:
Human
Mouse
Rat
Bovine
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Brand Family:
Upstate
Product Name:
Anti-Citrulline (Modified) Detection Kit
Components:
Anti-Citrulline (Modified)
Goat Anti-Rabbit IgG, HRP
1% Ovalbumin
Reagent B
0.5% FeCl3
20X TBS
View All »
Host:
Rabbit
Packaging:
Kit capacity: 10 western blots |