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Rap1 Activation Assay Kit
Description:
Rap1 Activation Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
Key Applications:
Affinity Binding Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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UniProt Summary:
FUNCTION: SwissProt: P52306 # Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
SIZE: 607 amino acids; 66403 Da
SIMILARITY: SwissProt: P52306 ## Contains 5 ARM repeats.
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Brand Family:
Upstate
Gene Symbol:
RAP1GDS1
MGC118859
GDS1
RAP1
MGC118861
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Product Name:
Rap1 Activation Assay Kit
Quality Assurance:
Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
Components:
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rap1 Activation Lysis Buffer, 2X
Anti-Rap1
Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
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UniProt Number:
P52306
Packaging:
Kit capacity: 20-30 assays
Entrez Gene Number:
NM_021159.3 |