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Tyrosine Kinase Assay Kit, Colorimetric Detection
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PRODUCT FAMILY INFORMATION
Phosphotyrosine Antibodies
Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
Millipore’s phosphotyrosine antibody demonstrates specificity against Phosphotyrosine. See below for data and references for Millipore’s phosphotyrosine antibodies. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Phosphotyrosine Signaling Solutions
Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
» More…
Description:
Tyrosine Kinase Assay Kit, Colorimetric Detection
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu4-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn2+/Mg2+ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the well of a streptavidin-coated 96-well plate. The second stage involves the detection of phosphorylated substrate by Direct Enzyme Linked Immunosorbent Assay (ELISA) using a monoclonal anti-phosphotyrosine-HRP (Horse Radish Peroxidase) antibody conjugate. Tetramethylbenzidine (TMB) is provided as the HRP substrate.
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Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Quality Assurance:
Routinely evaluated by a reference peptide titration. Increasing amounts of Poly (Glu4-phospho-Tyr) Peptide, biotinylated were bound to streptavidin-coated wells. Total peptide mass/well was adjusted by the addition of Poly (Glu4-Tyr) Peptide, biotinylated to a well. Bound phospho-tyrosine containing peptide was detected with Anti-Phosphotyrosine, recombinant 4G10, HRP conjugate.
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Components:
Streptavidin Coated Microwell Plate (Cat.# 20-183)
Anti-pTyr, recombinant 4G10™, HRP
ELISA wash and blocking buffers
Poly (Glu4-Phospho-Tyr), biotinylated
Poly (Glu4-Tyr), biotinylated
Tyrosine Kinase Reaction Buffer, 5X
Sodium Orthovanadate, 50mM
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Brand Family:
Upstate
Promotional Text:
Special Offer on Antibodies! Click Here!
Detection Methods:
Colorimetric
Colorimetric
Product Name:
Tyrosine Kinase Assay Kit, Colorimetric Detection
Analytes Available:
Tyrosine Kinase |