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Rac1 cDNA Allelic Pack
Description:
Rac1 cDNA Allelic Pack
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Key Applications:
Transfection
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
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UniProt Summary:
FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
SIZE: 192 amino acids; 21450 Da
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
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Quality Assurance:
This kit contains transfection grade eukaryotic expression vectors containing wild type, activated, and dominant negative human Rac1 alleles under the control of the CMV promoter and the empty vector control, for use in either transient or stable transfection of mammalian cells.
Components:
Rac1 cDNA (wt) in pUSEamp (Cat.# 21-200)
Rac1 cDNA (activated) in pUSEamp (Cat.# 21-193)
Rac1 cDNA (dominant negative) in pUSEamp (Cat.# 21-199)
pUSEamp (+) (Cat.# 21-147)
Brand Family:
Upstate
UniProt Number:
P63000
Gene Symbol:
RAC1
MGC111543
ORFNames=MIG5
PAK1
MIG5
TC-25
p21-Rac1
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Product Name:
Rac1 cDNA Allelic Pack
Entrez Gene Number:
NM_006908.3< |