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p300/CBP Immunoprecipitation HAT Assay Kit
Description:
p300/CBP Immunoprecipitation HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
The p300/CBP Immunoprecipitation HAT Assay Kit contains nonradioactive components for assessing the histone acetyl transferase (HAT) activity of
immunoprecipitated p300/CBP.
Key Applications:
Histone Acetyltransferase Assay (HAT)
Species Reactivity:
Human
Mouse
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
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UniProt Summary:
FUNCTION: SwissProt: Q92793 # Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes.
SIZE: 2442 amino acids; 265351 Da
SUBUNIT: Found in a complex containing NCOA2; NCOA3; IKKA; IKKB and IKBKG. Probably part of a complex with HIF1A and EP300. Interacts with phosphorylated CREB1. Interacts with the C-terminal region of CITED4. The TAZ-type 1 domain interacts with HIF1A. Interacts with SRCAP, CARM1, ELF3, MLLT7/FOXO4, N4BP2, NCOA1, NCOA3, NCOA6, PCAF, PELP1, PML, SMAD1, SMAD2, SMAD3, SPIB and TRERF1. Interacts with HTLV-1 Tax and p30II. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN:SwissProt: Q92793 The KIX domain mediates binding to HIV-1 Tat.
PTM: Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: Q92793 # Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. & Defects in CREBBP are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
SIMILARITY: Contains 1 bromo domain. & Contains 1 KIX domain. & Contains 2 TAZ-type zinc fingers. & Contains 1 ZZ-type zinc finger.
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Brand Family:
Upstate
Gene Symbol:
CREBBP
CBP
RTS
RSTS
Product Name:
p300/CBP Immunoprecipitation HAT Assay Kit
Alternate Names:
KAT3A
KAT3B
Quality Assurance:
Routinely evaluated by immunoprecipitating HeLa nuclear extract with either normal mouse IgG or anti- p300. The immune complexes were subjected to a HAT assay using Histone H4 Peptide, biotinylated
Components:< |