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ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set
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PRODUCT FAMILY INFORMATION
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
EMD Millipore’s Histone H4 antibody demonstrates specificity against linker histone H4. See below for data and references for EMD Millipore’s Histone H4 antibodies and proteins. All EMD Millipore antibodies and proteins are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H, Vrt IP, WB, ChIP Rabbit Purified Polyclonal Antibody
Description:
ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H4 (Lys16) set includes the Acetyl-Histone H4 (Lys16) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 178 bp region of human RPL10 promoter. The Acetyl-Histone H4 (Lys16) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H4 (Lys16)-associated chromatin.
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Specificity:
This antibody detects histone H4 acetylated at Lys16.
Molecular Weight:
~10 kDa observed
Epitope:
Acetylated H4 Lys16
Immunogen:
KLH-conjugated Histone H4 corresponding to the N-terminus region at and around acetylated Lys16.
Modifications:
Acetylation
Background Information:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
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Species Reactivity:
Human
Vertebrates
Species Reactivity Note:
Human. Broad species cross-reactivity is expected based on sequence homology.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µg of Normal rabbit IgG or 2 µL of Anti-acetyl-Histone H4 (Lys16) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of acetyl-Histone H4 (Lys16) associated DNA fragments was verified by qPCR using ChIP Primers, RPL10 Promoter as a positive locus, and β-Globin primers as a negative locus. (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
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