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Magna ChIP2 ™ Universal ChIP DNA Microarray Quad Kit
Description:
Magna ChIP2 ™ Universal Chromatin Immunoprecipitation DNA Microarray Quad Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
12 assays
Product Overview:
ChIP-chip (ChIP-on-chip) is a high content approach that combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to analyze how regulatory and structural proteins, such as transcription factors and histone subunits, bind and interact with the genome of living cells. Through the immunoprecipitation of these protein-DNA complexes, associated sequences are pulled out of solution for analysis on DNA microarrays. Using high quality antibodies, post-translational modifications to these sequences can also be detected.
The combination of ChIP and promoter or genomic tiling microarrays allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution at a variety of locations. This more comprehensive approach allows for greater insight into gene regulatory networks and can facilitate the creation of genome-wide maps of protein-DNA interactions or histone modifications.
Millipore’s Magna ChIP2 ™ Chromatin Immunoprecipitation DNA microarray kits are designed to help address these challenges and make ChIP-chip technology easily accessible to a wider range of laboratories. Each of these user-friendly kits provides a set of optimized reagents and validated protocols to facilitate ChIP-chip for both beginners as well as experienced users.
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Applications:
The ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG) & qPCR primers which amplify a 165 bp region of human H19 internal control region.
Key Applications:
ChIP-chip
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Brand Family:
Upstate
Presentation:
Nine boxes containing all necessary reagents to perform the chromatin immunoprecipitation and amplification steps for 12 ChIP-on-chip assays/ comparison reactions.
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Product Name:
Magna ChIP2 ™ Universal Chromatin Immunoprecipitation DNA Microarray Quad Kit
Materials Required but Not Delivered:
Labeling and hybridization reagents.
User-provided microarray slides.
Magna Grip™ Rack 8 well (Cat.# 20-400) (Now Available!) or similar magnetic rack.
Components:
Chromatin IP Module I (x4)
Chromatin IP Module II (x4)
Chromatin Amplification Module (x1)
Packaging:
Magna ChIP2 Kit capacity: 12 ChIP-on-chip assays |