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Universal Phosphotyrosine Detection HTRF® Assay
Description:
Universal Phosphotyrosine Detection HTRF® Assay
Qty/Pk:
1 plate
Product Overview:
The Universal Phosphotyrosine Detection HTRF® Assay (Cat. # 17-10015) is a complete homogenous method for measuring tyrosine kinase activities using the industry standard monoclonal phosphotyrosine antibody, 4G10®. Clone 4G10 has been validated by thousands of researchers to detect phosphorylation of virtually every tyrosine target. The kit includes Clone 4G10 conjugated to Europium, a tyrosine peptide substrate and a universal detection system, HTRF®. An increase in the HTRF® signal is observed as a result of the kinase activity. Inhibitor compounds can be screened during the kinase reaction for an IC50 value determination.
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Background Information:
Tyrosine Phosphorylation Protein phosphorylation is often necessary for the initiation of biological processes such as cell growth, proliferation, and ubiquitin mediated degradation. Tyrosine phosphorylation, in particular, often mediates receptor signaling events at the membrane by initiating protein-protein interactions in response to growth factors, hormones and cytokines.
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Applications:
The Universal Phosphotyrosine Detection HTRF Assay is a complete homogenous method for measuring tyrosine kinase activities using the industry standard monoclonal phosphotyrosine antibody, 4G10.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
Black 96 well plate: (Part No. R5013) One 96-well black plate.
Detection buffer: (Part No. CS206478) One bottle containing 5 mL of detection buffer.
10X KinEASE buffer: (Part No. CS206508). 1 vial containing 1 mL of kinase reaction buffer.
Biotinylated TK1 Peptide: (Part No. CS206504). 1 vial containing 60 µL of Biotinylated TK1 Peptide Stock at 50 µM.
Biotinylated TK2 Peptide: (Part No. CS206505). 1 vial containing 60 µL of Biotinylated TK2 Peptide Stock at 50 µM.
Biotinylated TK3 Peptide: (Part No. CS206506). 1 vial containing 60 µL of Biotinylated TK3 Peptide Stock at 50 µM.
Biotinylated Phosphotyrosine Peptide Standard: (Part No. 90192). One vial containing 10 µg lyophilized biotinylated phosphotyrosine peptide standard.
Streptavidin-APC: (Part No. CS206501). 1 vial containing 360 µL of streptavidin-APC stock at 1 µM.
Eu-cryptate-conjugated 4G10 anti-phosphotyrosine: (Part No. CS206507). 1 vial containing 30 µL Eu-cryptate conjugated 4G10 anti-phosphotyrosine antibody stock at 35 µg/mL.
ATP: (Part No. CS206509). 1 vial containing 50 µL of ATP stock at 10mM.
DTT: (Part No, 90499). 1 vial containing 100 µL of DTT stock at 1M.
MnCl2: (Part No. CS206510). 1 vial containing 100 µL of MnCl2 stock at 0.1M.
MgCl2: (Part No. CS206511). 1 vial containing 100 µL of MgCl2 stock at 1M.
Sodium Orthovanadate: (Part No. CS206465). 1 vial containing 50 µL of sodium orthovanadate stock at 50 mM.
Staurosporine: (Part No. CS201778). One vial containing 100 µL of staurosporine stock at 2.5 mM.
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Detection Methods:
HTRF
Packaging:
kit capacity: 96 wells
Product Name:
Universal Phosphotyrosine Detection HTRF® Assay
Analytes Available:
Tyrosine Kinase |