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Anti-CD45 Antibody, clone F10-89-4
Recommended Replacement for: 05-1410 is a recommended replacement for CBL124
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REFERENCES
The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration.
Jian Yu,Aijun Wang,Zhenyu Tang,Jeffrey Henry,Benjamin Li-Ping Lee,Yiqian Zhu,Failei Yuan,Fengping Huang,Song Li (2012) Biomaterials.33
Regional heterogeneity in murine lung fibroblasts from normal mice or mice exposed once to cigarette smoke.
Olena Preobrazhenska,Joanne L Wright,Andrew Churg (2012) PloS one.7
Species Reactivity Key Applications Host Format Antibody Type
H, M, R FC, WB, IF, IP, IH(P) Mouse Affinity Purified Monoclonal Antibody
Description:
Anti-CD45 Antibody, clone F10-89-4
Replaces:
05-1410 is a recommended replacement for CBL124
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Specificity:
This antibody reacts with the leucocyte common antigen (LCA) complex to be found on lymphocytes, monocytes, granulocytes, thymocytes and malignant T and B cells. No reactivity has been observed with primary or metastatic carcinoma cells. Plasma cells or myeloma cells may have weak expression or be negative for this antigen.
The LCA comprises of at least 5 isoforms ranging from 180-220 kDa which are produced as a result of alternative splicing of exons A, B or C. CBL 124 recognizes epitopes common to
all of the isoforms.
Antigen distribution:
Thymocytes > 95%
Granulocytes > 95%
Monocytes > 95%
B cells (CD20+) > 95%
T cells (CD3+) > 95%
NK cells (CD16+) > 95%
Peripheral blood lymphocytes > 95%
FUSION PARTNER: NS1 myeloma cell line
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Molecular Weight:
~180-220 kDa
Immunogen:
Purified T cells from human lymph nodes
Clone:
F10-89-4
Isotype:
IgG2a
Background Information:
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Mouse. Expected to react with human and rat based on sequence homology.
Application Notes:
Immunofluorescence: A previous lot of this antibody was used in the identification of cells of leucocyte origin by indirect staining.
Immunohistochemistry (paraffin): A previous lot of this antibody was used in the discrimination between malignant cells of haematopoietic origin and other malignancies in frozen tissue sections.
Immunoprecipitation: A previous lot of this antibody was used in studies of the leucocyte common antigen complex and its identification by the Western blotting technique.
Flow Cytometry: Studies of CD45 expression and the association with T-cell receptor signaling was performed using this antibody of previous lot.
Studies of the mechanism by which CD45 regulates B cell activation through protein tyrosine phospatases.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of CD45 Monoclonal Antibody: Tonsil
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Control:
Jurkat cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on jurkat cell lysate.
Western Blot Analysis: 1:500 dilution of this lot detected CD45 on 10 μg of Jurkat lysat |