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Anti-monomethyl Histone H3 (Lys9), clone CMA306
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PRODUCT FAMILY INFORMATION
Histone H3 Antibodies, Proteins and Kits
Millipore’s Histone H3 antibodies demonstrate specificity against histone H3. See below for acetyl-, methyl-, phospho- histone H3 Antibodies and Proteins, based on the expertise of Upstate & Chemicon.
Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Species Reactivity Key Applications Host Format Antibody Type
H WB, PIA Mouse Purified Monoclonal Antibody
Description:
Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306
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Specificity:
This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.
Molecular Weight:
~17 kDa
Epitope:
monomethylated H3 Lys9
Immunogen:
KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.
Clone:
CMA306
Isotype:
IgG2a
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
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Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with human.
Application Notes:
Dot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.
Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
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Control:
Recombinant Histone H3 and HeLa acid extract.
Quality Assurance:
Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of receipt.
UniProt Number:
O43463
Entrez Gene Number:
NP_003484
Gene Symbol:
EC 2.1.1.43
KMT1A
MG44
OTTHUMP00000024298
SUV39H
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Alternate Names:
H3K9me1
Histone H3 (mono methyl K9)
H3-K9-HMTase 1
Histone H3-K9 methyltransferase 1
Lysine N-methyltransferase 1A
Position-effect variegation 3-9 homolog
Su(var)3-9 homolog 1
Suppressor of variegation 3-9 homolog 1
histone-ly |