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Anti-ILK, clone 6G6.3
Recommended Replacement for: 05-575
Species Reactivity Key Applications Host Format Antibody Type
M, H, R WB Mouse Purified Monoclonal Antibody
Description:
Anti-ILK Antibody, clone 6G6.3
Replaces:
05-575
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes ILK
Molecular Weight:
51 kDa
Immunogen:
Full length recombinant GST-tagged human ILK.
Clone:
6G6.3
Isotype:
IgG3κ
Background Information:
ILK (Integrin-Linked Kinase) is an intraceullar Ser/Thr kinase with four Ankyrin-like repeats. It is known to play pivotal roles in extracellular matrix mediated signaling. ILK is known to associate with β-Integrins in addition to many other focal adhesion associated proteins. In this context, ILK is involved cell adhesion/migration. The kinase activity appears to be reduced following Integrin activation, and overexpression of p59ILK inhibits cellular adhesion to Integrin substrates. Additionally, ILK has been associated with epithelial mesechymal transition (EMT) in a TGF-β1 induced Smad dependent manner (Li, 2003). Forced expression of ILK also induced the expression of MMP-2 (matrix metalloprotease 2) and promoted cell migration and invasion (Li, 2003).
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Species Reactivity:
Mouse
Human
Rat
Application Notes:
Not recommended for IP
Quality Assurance:
Routinely evaluated by western blot on HEK293 cell lysate.
Purification Method:
Protein G purfied
Presentation:
Protein G purified mouse monoclonal in storage buffer containing 0.1M Tris-Glycine, pH7.4, 15 mM NaCl, and 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.
UniProt Number:
Q13418
Entrez Gene Number:
NP_004508.1
Gene Symbol:
ILK
p59ILK
ILK-1
P59
ILK1
ILK-2
ILK2
DKFZp686F1765
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Alternate Names:
Integrin-Linked Kinase
p59
DKFZp686F1765
p59ILK
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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UniProt Summary:
FUNCTION: SwissProt: Q13418 # Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PI |