|
Anti-phospho-Met (Tyr1234/1235), clone NL8
Recommended Replacement for: 05-900
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit null Monoclonal Antibody
Description:
Anti-phospho-Met (Tyr1234/1235) Antibody, clone NL8 | 04-900
Replaces:
05-900
Promotional Text:
Special Offer on Antibodies! Click Here!
Specificity:
Recognizes phosphorylated Met, Mr 140 kDa. Cross-reacts with phosphorylated EGFR.
Molecular Weight:
~140 kDa
Epitope:
pTyr1234/1235
Immunogen:
KLH-conjugated, synthetic peptide (…KE[pY][pY}SV…) in which pYpY corresponds to phosphorylated amino acids 1234 and 1235 of human Met/HGFR.
Modifications:
Phosphorylation
Clone:
NL8
Isotype:
IgG
Background Information:
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
View All »
Species Reactivity:
Human
Species Reactivity Note:
Human
Control:
RIPA lysates from A431 cells co-expressing Met/HGFR and HGF.
Quality Assurance:
Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-Met in RIPA lysates from NIH-3T3 cells co-expressing Met/HGFR and HGF.
Presentation:
Cultured supernantant containing 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
UniProt Number:
P08581
Entrez Gene Number:
NP_000236.2
Gene Symbol:
AUTS9
EC 2.7.10.1
HGFR
OTTHUMP00000069168
RCCP2
c-Met
View All »
Alternate Names:
HGF receptor
HGF/SF receptor
Met proto-oncogene tyrosine kinase
SF receptor
Scatter factor receptor
met proto-oncogene
met proto-oncogene (hepatocyte growth factor receptor)
oncogene MET
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various
mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants
encoding different isoforms have been found for this gene.
View All »
Key Applications:
Western Blottin |