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Anti-phospho CD22 (Tyr807) Antibody, rabbit monoclonal
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REFERENCES
Genomic structure and chromosomal mapping of the human CD22 gene.
Wilson, G L, et al. (1993) J. Immunol., 150: 5013-24 (1993)
A novel subset of murine B cells that expresses unmasked forms of CD22 is enriched in the bone marrow: implications for B-cell homing to the bone marrow
Floyd, H, et al (2000) Immunology, 101:342-7 (2000)
Species Reactivity Key Applications Host Format Antibody Type
Human Only WB Rabbit Unpurified Monoclonal Antibody
Description:
Anti-phospho CD22 (Tyr807) Antibody, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes a region composed of residues surrounding Tyrosine 807 of human CD22.
Immunogen:
KLH-conjugated synthetic phospho-peptide composed of residues surrounding Tyrosine 807 of human CD22.
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human Only
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
In 60% storage buffer (50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
Storage Conditions:
2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
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UniProt Number:
P20273
Entrez Gene Number:
NM_001771.1
Gene Symbol:
CD22
SIGLEC-2
MGC130020
Leu-14
SIGLEC2
Siglec-2
BL-CAM
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P20273 # Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
SIZE: 847 amino acids; 95348 Da
SUBUNIT: Predominantly monomer of isoform CD22-beta. Also found as heterodimer of isoform CD22-beta and a shorter isoform. Interacts with PTPN6/SHP-1, LYN, SYK, PIK3R1/PIK3R2 and PLCG1 upon phosphorylation. Interacts with GRB2, INPP5D and SHC1 upon phosphorylation (By similarity). May form a complex with INPP5D/SHIP, GRB2 and SHC1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: B-lymphocytes.
DOMAIN: SwissProt: P20273 Contains 4 copies of a cytoplasmic motif that is referred |