|
Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3
Close
REFERENCES
c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function
Biscardi, J S, et al (1999) J Biol Chem, 274:8335-43 (1999)
Phosphorylation of Y845 on the epidermal growth factor receptor mediates binding to the mitochondrial protein cytochrome c oxidase subunit II
Boerner, Julie L, et al (2004) Mol Cell Biol, 24:7059-71 (2004)
Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines
Moro, Laura, et al (2002) J Biol Chem, 277:9405-14 (2002)
Species Reactivity Key Applications Host Format Antibody Type
H, M ELISA, ICC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes phosphorylated EGFR on Tyrosine 845.
Molecular Weight:
180 kDa
Immunogen:
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 845 in human EGFR.
Modifications:
Phosphorylation
Clone:
12A3
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Control:
Includes EGF treated Hep2G cell lysate as a positive control
Quality Assurance:
Routinely evaluated by immunoblot.
Purification Method:
Subsequent thiophilic adsorption and size exclusion chromatography
Presentation:
100 µg of mouse monoclonal IgG1 lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose
Storage Conditions:
2 years at -20°C from date of shipment
UniProt Number:
P00533
Entrez Gene Number:
NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1
Gene Symbol:
EGFR
ERBB1
ERBB
mENA
Alternate Names:
ERBB; ERBB1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Immunocytochemistry
Immunoprecipitation
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase super |