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Anti-phospho-c-Jun (Ser63),clone Y172
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Species Reactivity Key Applications Host Format Antibody Type
H, M, R ICC, IHC, IH(P), IP, WB Rabbit Culture Supernatant Monoclonal Antibody
Description:
Anti-phospho-c-Jun (Ser63) Antibody,clone Y172, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Specificity:
This antibody recognizes phosphorylated c-Jun at and around Ser63.
Molecular Weight:
36-42 kDa
Epitope:
Ser63
Immunogen:
Synthetic peptide directed against phosphoylated Ser63 of human c-Jun and surrounding amino acids.
Modifications:
Phosphorylation
Clone:
Y172
Isotype:
IgG
Background Information:
Jun is a component of the transcription factor AP-1, and can constitute AP-1 activity either as a homodimer or as a heterodimer with Fos. By contrast with Fos, Jun is expressed constitutively, and can be activated by phosphorylation of serines 63 and/or 73. These sites are targets for the SAPK1/JNK family of protein kinases, and their phosphorylation states can be monitored using antibodies specific for the phospho-epitopes. In addition, Jun is phosphorylated by GSK3 on sites which inhibit Jun activity. Since GSK3 is inactivated by Akt, the Akt pathway results in Jun activation.
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Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Proven to react with human, mouse and rat.
Application Notes:
Immunohistochemistry: A 1:50-100 dilution of a previous lot was used in IH.
Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.
Immunocytochemistry: A 1:100-200 dilution of a previous lot was used in IC.
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Control:
Anisomycin treated NIH/3T3 cell lysate
Quality Assurance:
Routinely evaluated by Western Blot on NIH/3T3 cell lysate.
Western Blot Analysis: A 1:5,000-1:10,000 dilution of this lot was used to detect Phospho c-Jun (Ser63) NIH/3T3 cell lysate.
Presentation:
Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
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UniProt Number:
P05412
Entrez Gene Number:
NP_002219.1
Gene Symbol:
AP-1
AP1
OTTHUMP00000010036
c-Jun
p39
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Alternate Names:
Activator protein 1
Jun activation domain binding protein
Proto-oncogene c-jun
V-jun avian sarcoma virus 17 oncogene homolog
enhancer-binding protein AP1
jun oncogene
v-jun sarcoma virus 17 oncogene homolog
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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