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产品参数：
MW: 2309.6
Purity: 95%
Appearance: Lyophilized solid.
Solubility: Soluble in Water.
Long Term Storage: -20°C
产品说明：
The peptide sequence, PHCVPRDLSWLDLEANMCLP, named R18, was identified as an unphosphorylated peptide that binds to 14-3-3 proteins with high affinity (Kd=80 nM). It has been shown to bind to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides. R18 can inhibit the formation of the 14-3-3-Raf-1 complex, reduce Raf-1 dependent transcriptional activity in cells and block the activation of ExoS by 14-3-3. It binds to all the 14-3-3 isoforms with equal affinities. This peptide is useful for competitive inhibition experiments.
A biotinylated form is available (BML-P225), allowing detection using streptavidin.
这种肽序列,PHCVPRDLSWLDLEANMCLP,名叫R18,被确认为一个unphosphorylated肽,绑定到14-3-3蛋白具有高亲和力(Kd = 80海里)。它已经被证明可以绑定到相同的两亲槽表面上的14-3-3作为磷酸化肽。R18可以抑制地层的14 3 3皇家空军1复杂,减少皇家空军1依赖转录活动在细胞和块的激活ExoS 14-3-3。它将与所有的14-3-3亚型以同样的亲和力。这种肽是有用的竞争性抑制实验。
一个生物酰化的形式是可用的(BML-P225),允许检测使用链霉亲和素。 |