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Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0.5µl or less per well is sufficient for assay with the HDAC Fluor de Lys™ fluorometric activity assay (Prod. No. BML-AK500).
Product Specification
Formulation:Liquid. In 0.1M KCl, 20mM HEPES/NaOH, pH 7.9, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF.
Long Term Storage:-80°C |