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Ac-LEVD-AFC
Caspase-4 substrate
Fluorogenic substrate for caspase-4. Similar to the fluorogenic substrate Ac-LEVD-AMC (Prod. No. ALX-260-083); the AFC fluorophore is more sensitive.
Product Specification
Alternative Name: Caspase-4 substrate (fluorogenic)
Sequence: Ac-Leu-Glu-Val-Asp-AFC (AFC=7-Amido-4-trifluoromethylcoumarin)
Formula: C32H40F3N5O11
MW: 727.7
Peptide Content: 75-95%.
Purity: ≥98% (HPLC)
Appearance: White to off-white powder.
Solubility: Soluble in DMSO; dilute with distilled water or buffer; also soluble in phosphate buffer, pH 7.5-8.0.
Long Term Storage: -20°C
Use/Stability: Dissolve only as much as needed at one time because of the possibility of decomposition in solution. Store solution at -20°C. Protect from condensation: when taking the sample out of the container, wait until it warms up to room temperature, then open it.
Handling: Keep cool and dry.
Background / Technical Information: AFC has an excitation maximum of 400nm and an emission maximum of 505nm. |