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Ac-WEHD-pNA
Substrate for caspase-1, -4- and -5
Replaces Prod. #: BML-P451
Colorimetric caspase substrate. Combinatorial studies have found the WEHD sequence to be optimal for clevage of tetrapeptide substrate by caspase-1, caspase-4, and caspase-5. Cleavage of the substrate by a caspase releases pNA, which can be detected by its absorbance at 405nm (
Product Specification
Alternative Name: Caspase-1 substrate (chromogenic), Caspase-5 substrate (chromogenic)
Sequence: Ac-Trp-Glu-His-Asp-pNA (pNA=p-Nitroaniline)
Formula: C34H37N9O11
MW: 747.7
Formulation: Lyophilized.
Peptide Content: 70-90%
Purity: ≥98% (HPLC)
Appearance: Yellow powder.
Solubility: Soluble in DMSO. Dilute with buffer, pH 7.5.
Long Term Storage: -20°C
Handling: Keep cool and dry.
Background / Technical Information: λmax of pNA is 400nm. |