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MALP-2 (BULK)
TLR activator
MALP-2 was originally isolated from Mycoplasma fermentans. This MALP-2 corresponds to the originally isolated isomer, which expresses potent endotoxin-like activity and approaches in certain experimental systems the toxicity of LPS. For description of the stereochemistry of MALP-2. MALP-2 signalling, unlike that of LPS, is not transduced via TLR4, but is induced via TLR2 and TLR6 signalling. BULK size (500μg) of biologically active, pyrogen-free and sterile MALP-2 for in vitro and animal in vivo studies.
Product Specification
Alternative Name: Macrophage-activating lipopeptide-2, S-[2,3-bis(Palmityloxy)-(2R)-propyl-cysteinyl-GNNDESNISFKEK]
Biological Activity: Stimulates macrophages, monocytes and other TLR2 and TLR6 expressing cells in vitro at pg/ml concentrations (e.g. mouse macrophages: 50pg/ml; fibroblasts: 2ng/ml) to release various mediators. Human monocytes require about 10-100x higher concentrations for optimal cytokine or chemokine release than mouse PECS. In vivo active at 0.5-5μg (e.g 0.5μg/20g mouse). The presence of 50mM octyl glucoside as carrier gives rise to a higher activity.
Formula: C99H167N19O30S
MW: 2135.2
Source: Synthetic.
Concentration: 1mg/ml
Formulation: Liquid. Pyrogen-free, sterile solution in 33% (v/v) 2-propanol/water.
Purity Detail: HPLC purified
Hazard: TOXIC.
Short Term Storage: -20°C
Long Term Storage: -80°C
Use/Stability: Stable for at least 3 years when stored at -20°C. This stock solution is stable for many months when kept in the refrigerator. MALP-2 solutions in the presene of carrier (50mM octyl glucoside, see Background/Technical Information below) are stable at +4°C at least 6 weeks. Some (10%) decrease of activity may occur shortly after transfer into new vessels. This is not due to decomposition but to adsorption (see below). Storage at room temperature for short periods of time does not influence bioactivity. When deep-frozen, MALP-2 does not loose activity within years.
Handling: Avoid accidental injection, extreme care should be taken with hypodermic syringes. Avoid inhalation and prevent this compound from entering the bloodstream. Avoid freeze/thaw cycles.
Background / Technical Information: MALP-2 sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2 stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2 is sensitive to alkali or acid. The medium should be preconditioned in the CO2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate. |