|
PDGF-BB, human recombinant
A growth factor that regulates cell growth and division
Product Overview
Alternate Names / Synonyms: Human PDGF-BB, h-PDGF-BB, PDGFBB, PDGF BB, PDGF-BB, rh-PDGF-BB, recombinant human PDGF-BB, recombinant PDGF-BB, PDGF
Gene Symbol: PDGFB
Accession #: P01127
Gene ID: 5155
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives
Molecular Weight: 24.3 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of thimidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of > 1 x 10⁶ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3 kDa B chain homodimer protein formed by subunits of 109 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |