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Leptin, human recombinant
Adipose-derived hormone which regulates energy intake & energy expenditure
Product Overview
Alternate Names / Synonyms: Human Leptin, h-Leptin, rh-Leptin, recombinant human Leptin, recombinant Leptin
Gene Symbol: LEP
Accession #: P41159
Gene ID: 3952
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized
Molecular Weight: 16.0 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: BioVision’s human Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 mg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
Reconstitution Instructions: Centrifuge the vial prior to opening. Add 3 mM acetic acid at a final concentration of 5-10 mg/ml. After the protein is completely dissolved, it can be diluted in other buffers for use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Human Leptin is a 16 kDa protein containing 146 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |