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IL-12, rat recombinant
A T cell-stimulating factor
Product Overview
Alternate Names / Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12
Gene Symbol: IL12a, IL12b
Accession #: NP_072133
Gene ID: 84405, 64546
Source: Baculovirus (Sf9 insect cells)
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 1 mg/ml solution with no additives.
Molecular Weight: 70 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: ≥95%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ range is 0.01-0.05 ng/ml, as determined by the dose-dependent secretion of IFN-γ from PHA and IL-12 activated mouse splenocytes that has been co-stimulated with PMA.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to dissolve). The solution can be diluted into other aqueous buffers
Storage Temp.: -20°C
Shipping: Blue ice
Background Information: The recombinant rat IL-12 produced from Sf9 insect cells is a glycosylated disulfide linked hetromeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa and 40 kDa subunits
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |