Human IL-12

IL-12, human recombinant
A T cell-stimulating factor
Product Overview
Alternate Names / Synonyms: Human IL-12, IL-12, IL12, IL 12, h-IL-12, rh-IL-12, recombinant human IL-12, recombinant IL-12, IL, interluekin
Gene Symbol: IL12
Accession #: P29459
Gene ID: 3592
Source: CHO cells
Appearance: Lyophilized protein
Physical Form Description: Sterile filtered and lyophilized in 0.5X PBS, pH 7.2
Molecular Weight: 75.0 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the induction of IFN-g production from murine splenocytes co-stimulated with IL-12 is <0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁷ units/mg.
Reconstitution Instructions: Reconstitute in 1X PBS (pH 7.2-7.4) to a concentration of 0.2 mg/ml. The solution can then diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IL-12 is a potent regulator of cell mediated immune responses and in induces IFN- production by NK and T cells. It is produced by activated monocytes/macrophage cells, B-lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induce the development of IFN- producing Th1 cells. Recombinant human IL-12 is a 75 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.