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IL-6, murine recombinant
An important mediator of acute phase response
Product Overview
Alternate Names / Synonyms: Murine IL-6, IL-6, IL6, IL 6, m-IL-6, rm-IL-6, recombinant murine IL-6, recombinant IL-6, IL, interluekin
Gene Symbol: IL6
Accession #: P08505
Gene ID: 16193
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives.
Molecular Weight: 21.7 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of the IL-6 dependent murine 7TD1 cells is <0.02 ng/ml, corresponding to a specific activity of >5 x 10⁷ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant murine IL-6 is a 21.7 kDa protein containing 187 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |