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IL-4, murine recombinant
A key regulator in humoral and adaptive immunity
Product Overview
Product Name: IL-4, murine recombinant
Alternate Name/Synonyms: BCGF,BCDF, B cell stimulating factor, BSF-1,Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra,
Gene Symbol: IL4
Accession #: P07750
Gene ID: 16189
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized with no additives.
Molecular Weight: 13.5 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ determined by the dose-dependent stimulation of the proliferation of murine HT-2 cells is <.2.0 ng/ml, corresponding to a specific activity of >5 x 10⁵ units/mg.
Reconstitution Instructions: Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant murine IL-4 is a 13.5 kDa globular protein containing 120 amino acid residues.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |