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IL-1beta, murine recombinant
An important mediator of inflammatory responses
Product Overview
Alternate Names / Synonyms: Murine IL-1beta, IL-1beta, IL 1beta,IL1beta, m- IL-1beta, rm-IL-1beta, recombinant murine IL-1beta, recombinant IL-1beta, IL, interluekin
Gene Symbol: IL1B
Accession #: P10749
Gene ID: 16176
Source: E. coli
Appearance: Lyophilized protein
Physical Form Description: Lyophilized from 5 mM Tris, pH 8.0
Molecular Weight: 17.5 kDa
Purity by SDS-PAGE: ≥98%
Purity by HPLC: ≥98%
Endotoxin Level: <0.1 ng/μg
Biological Activity: The ED₅₀ determined by the dose-dependent stimulation of murine D10S cells is <0.002 ng/ml, corresponding to a specific activity of >5 x 10⁸ units/mg.
Reconstitution Instructions: Reconstitute in water to a concentration of 0.1-1 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant murine IL-1β is a 17.5 kDa protein containing 153 amino acid residues.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans. |