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Green Fluorescence Protein (GFP) Antibody (Clone BV-F4)
Product Overview
Antibody Target: GFP
Alternative Name: Green Fluorescent Protein
Host: Mouse
Antibody Type: Monoclonal (Clone BV-F4)
Isotype: Mouse IgG1
Immunogen: Recombinant EGFP expressed from E. col
Accession#: ABG78037
Gene ID: 110612126
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: all
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |