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APG10/ATG10 Antibody
Product OverviewAntibody Target: APG10/ATG10
Alternative Name: APG10 , APG10L, ATG10 , DKFZP586I0418 , FLJ13954 , pp12616
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide, near the carboxyl terminus of human APG10
Accession#: EAW95884
Gene ID: 84734
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 0.5 mg/ml affinity purified rabbit anti-APG10 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 (Also called ATG10, APG10L), an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |