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APG7/ATG7 Antibody
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Product OverviewAntibody Target: APG7/ATG7
Alternative Name: APG7L , ATG7 , DKFZp434N0735 , GSA7
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide near the N-terminus of rat APG7
Accession#: NP_001012097
Gene ID: 312647
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 0.5 mg/ml affinity purified rabbit anti-APG7/ATG7 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.
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