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15-hydroxy Prostaglandin Dehydrogenase (human recombinant)
Cayman Chemical Item Number 10007950
Description
Source:active human recombinant N-terminal GST-tagged protein expressed in E. coli · Mr:~55 kDa · NAD+-dependent 15-hydroxy prostaglandin dehydrogenase (15-PGDH) catalyzes the oxidation of PGs to 15-keto metabolites that have greatly reduced biological activity.1↗ Human NAD+-dependent 15-PGDH is a 266 amino acid protein with a molecular weight of 29 kDa and is expressed in multiple tissues including lung, placenta, and kidney.2↗ The enzyme belongs to a large family of short-chain alcohol dehydrogenases, which exhibit amino acid identity ranging from 15-30%.3↗,4↗ Recent studies indicate 15-PGDH may play a role as a tumor suppressor. Studies have shown an inverse correlation between 15-PGDH expression and cell proliferation in colon, breast, and thyroid cells.5↗,6↗,7↗ 15-PGDH specific activity was determined using PGE2 as the substrate and NAD+ as the cofactor and was calculated after subtracting the mass contribution form the GST tag. The reaction was inhibited using CAY10397.
Synonyms 15-hydroxy PGDH
Formulation A solution in 50 mM Tris-HCl, pH 7.5, containing 1 mM EDTA, 1 mM DTT, and 30% glycerol
Purity ≥95% by SDS-PAGE
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere |