|
Thioredoxin Reductase Colorimetric Assay Kit
Cayman Chemical Item Number 10007892
Description
Cayman’s Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from tissue homogenates and cell lysates in a 96-well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm.1↗,2↗ The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.3↗
Synonyms TrxR Assay Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere |