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Monoacylglycerol Lipase (human recombinant)
Cayman Chemical Item Number 10007812
Description
Source:active human recombinant C-terminal His-tagged protein expressed in E. coli · Mr:34 kDa · Endocannabinoids such as 2-arachidonoyl glycerol (2-AG) and arachidonyl ethanolamide (AEA) are biologically active lipids that are involved in a number of synaptic processes including activation of cannabinoid receptors. Monoacylglycerol lipase (MGL) is a serine hydrolase responsible for the hydrolysis of 2-AG to arachidonic acid and glycerol, thus terminating its biological function.1↗,2↗ MGL is the principal 2-AG hydrolase in the mammalian brain accounting for greater than 80% of the total 2-AG hydrolase activity.3↗ Human recombinant MGL was characterized using 4-nitrophenylacetate as a substrate, which upon hydrolysis by MGL, froms 4-nitrophenol with an absorbance maximum at 412 nm.
Synonyms MGL MAGL
Formulation 50 mM Hepes, pH 7.4, containing 100 mM sodium chloride, 5 mM magnesium chloride, 0.1% Trition, and 25% glycerol
Purity >95%
Stability 6 months
Storage -80°C
Shipping ice in continental US; may vary elsew |