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DMPO Nitrone Adduct Polyclonal Antiserum
Cayman Chemical Item Number 10006170
Description
Antigen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N-oxide coupled to ovalbumin · Host: rabbit · Application(s): WB, ICC, and ELISA · Measurement of protein radicals is now possible with the development of the 'spin trap immunoassay' 1↗,2↗ Some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.1↗ Cayman’s DMPO Nitrone Adduct Polyclonal Antiserum is a significant improvement over traditional electron paramagnetic resonance (EPR) detection of protein radical adducts. Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.2↗
1 Detweiler, C.D., Deterding, L.J., Tomer, K.B., et al. Immunological identification of the heart myoglobin radical formed by hydrogen peroxide. Free Radic Biol Med 33(3) 364-369 (2002).
2 Ramirez, D.C., Chen, Y., and Mason, R.P. Immunochemical detection of hemoglobin-derived radicals formed by reaction with hydrogen peroxide: Involvement of a protein-tyrosyl radical. Free Radic Biol Med 34(7) 830-839 (2003).
Synonyms 5,5-Dimethyl-1-Pyrroline-N-Oxide Nitrone Adduct
Formal Name
Formulation 100 µl of rabbit anti-DMPO nitrone adduct polyclonal antiserum
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity Species independent |