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N-Decanoyl p-Nitroaniline
Cayman Chemical Item Number 10005851
Description
N-Decanoyl p-nitroaniline (DepNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1↗ FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 410 nm). This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.
1 Patricelli, M.P., and Cravatt, B.F. Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase. Biochemistry 40 6107-6115 (2001).
Synonyms DepNA
Formal Name N-(4-nitrophenyl)-decanamide
CAS Number 72298-63-6
Molecular Formula C16H24N2O3
Formula Weight 292.4
Formulation A crystalline solid
Purity ≥98%
λmax 204, 316 nm
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES CCCCCCCCCC(=O)Nc1ccc(cc1)[N](=O)O
InCHI Code 1S/C16H24N2O3/c1-2-3-4-5-6-7-8-9-16(19)17-14-10-12-15(13-11-14)18(20)21/h10-13H,2-9H2,1H3,(H,17,19)
InCHI Key OYQMGBPTCMSMAD-UHFFFAOYSA-N |