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Doppel Polyclonal Antibody
Cayman Chemical Item Number 10005517
Description
Antigen:human doppel amino acids 112-120 (ATQAANQGE) · Host:rabbit · Application(s):WB and IHC (formalin-fixed paraffin-embedded sections); other applications not tested · Doppel is a homolog of the cellular prion protein. Like prion protein, doppel has two N-linked oligosaccharides, and is presented on the cell surface via a glycosylphosphatidylinositol anchor.1↗ Unlike prion protein, it lacks the conformationally plastic and octapeptide repeat domains.2↗ The primary physiological role of doppel protein remains to be determined, but there is some evidence suggesting that cell surface prion protein can antagonize the toxic effect of doppel expressed in the central nervous system.1↗ In addition, the protein may play a major role in human male fertility, given its expression on both sertoli cells and spermatozoa.3↗ Cayman Chemical’s doppel polyclonal antibody can be used for western blot and immunohistochemistry (formalin-fixed paraffin-embedded sections) analysis on samples of human, mouse, or rat origin. The antibody recognizes the nonglycosylated and glycosylated forms of the protein.
Formal Name
Formulation
200 µg peptide affinity-purified IgG in 400 µl TBS, pH 7.4, containing 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity Human Doppel
Mouse Doppel
Rat Doppel |