DescriptionExonuclease-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3'-terminal phosphorothioate (PTO) modifications that are resistant to the 3'=>5' exonuclease activity of proofreading DNA polymerases (1), such as Klenow Fragment and phi29 DNA Polymerase. It also has 5'- and 3'-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.Features3’-terminal PTO modifications protect from 3’=>5’ exonuclease degradation and ensure efficient priming of DNA synthesis.Quality ControlFunctionally tested for the efficient priming of DNA synthesis using phi29 DNA Polymerase.ApplicationsStrand displacement amplification of genomic DNA (2), plasmids and phage DNA (3).DNA labeling by random priming (4-6).
