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Description
GeneRuler Express DNA Ladder, ready-to-use, is designed for fast separation (in 5-15 min at 23 V/cm) of double-stranded DNA fragments in agarose gels (seeTable 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler™ Express DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose gels.
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Features
Ideal for both DNA sizing and approximate quantification.
Ideal for fast separation under a wide range of electrophoresis conditions.
Sharp bands.
Bright reference bands (given in red).
Can be directly loaded and are stable at room temperature for 6 months.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
GeneRuler™ Storage and Loading Buffer (for ready-to-use ladders)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C. |