Description GeneRuler Express DNA Ladder is designed for fast separation (in 5-15 min at 23 V/cm) of double-stranded DNA fragments in agarose gels (seeTable 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler™ Express DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol. The ladder is supplied with 6X DNA Loading Dye for sample DNA. Quality Control Well-defined bands are formed during agarose gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by a direct nuclease activity assay. Features Ideal for both DNA sizing and approximate quantification. Ideal for fast separation under a wide range of electrophoresis conditions. Sharp bands. Bright reference bands (given in red). Supplied with loading dye for sample DNA. Storage Buffer (TE buffer) 10 mM Tris-HCl (pH 7.6) and 1 mM EDTA. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |