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Description
GeneRuler Express DNA Ladder is designed for fast separation (in 5-15 min at 23 V/cm) of double-stranded DNA fragments in agarose gels (seeTable 1). The band pattern of express ladders is not affected by the composition of electrophoresis buffer, voltage or gel percentage. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler™ Express DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Features
Ideal for both DNA sizing and approximate quantification.
Ideal for fast separation under a wide range of electrophoresis conditions.
Sharp bands.
Bright reference bands (given in red).
Supplied with loading dye for sample DNA.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |