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Description
GeneRuler High Range DNA Ladder is recommended for fast sizing and approximate quantification of high molecular weight double-stranded DNA on agarose gels (in 1.5 hour, 0.4% agarose gel). The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler High Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.)
The ladder is supplied with 6X DNA Loading Dye for sample DNA.
Features
Ideal for both DNA sizing and approximate quantification.
Ideal for fast sizing of high molecular weight DNA fragments
Sharp bands.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |