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Description
pBR322 DNA/BsuRI (HaeIII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pBR322 DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
Marker can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Features
Sizing and approximate quantification of small DNA fragments.
Sharp bands.
Supplied with loading dye for sample DNA.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |