Description phiX174 DNA/BsuRI (HaeIII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. phiX174 DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation. phiX174 DNA/BsuRI (HaeIII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols. Features Sizing and approximate quantification of small DNA fragments. Sharp bands. Supplied with loading dye for sample DNA. Quality Control Well-defined bands are formed during agarose gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by a direct nuclease activity assay. Storage Buffer (TE buffer) 10 mM Tris-HCl (pH 7.6) and 1 mM EDTA. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |