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Description
pUC19 DNA/MspI (HpaII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pUC19 DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
pUC19 DNA/MspI (HpaII) Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose and polyacrylamide gels.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Features
Sizing and approximate quantification of small DNA fragments.
Sharp bands.
Premixed with 6X DNA Loading Dye for direct loading and room temperature storage.
Supplied with loading dye for sample DNA. |