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进口原装|pUC19 DNA/MspI (HpaII) Marker

产品编号: SM0221     查看说明书
产品名称: 进口原装|pUC19 DNA/MspI (HpaII) Marker  .0   订购此产品 
供应商: MBI
规格: 50 μg
目录价: 441
库存状态: 现货
CAS编号:
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Description
pUC19 DNA/MspI (HpaII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pUC19 DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
pUC19 DNA/MspI (HpaII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Features
Sizing and approximate quantification of small DNA fragments.
Sharp bands.
Supplied with loading dye for sample DNA.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.

保存条件: -20℃
说明书地址: 点击查看详细
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