Description Lambda DNA/EcoRI+HindIII Marker, ready-to-use, is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker. Lambda DNA/EcoRI+HindIII Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose gels. Quality Control Well-defined bands are formed during agarose gel electrophoresis. The absence of nucleases is confirmed by a direct nuclease activity assay. Storage and Loading Buffer (for ready-to-use markers) 10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. Features Sizing and approximate quantification of large DNA fragments. Sharp bands. Premixed with 6X DNA Loading Dye for a direct loading (after heating) and for room temperature storage. Supplied with loading dye for sample DNA. |