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Description
Lambda DNA/EcoRI+HindIII Marker, ready-to-use, is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
Lambda DNA/EcoRI+HindIII Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose gels.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Features
Sizing and approximate quantification of large DNA fragments.
Sharp bands.
Premixed with 6X DNA Loading Dye for a direct loading (after heating) and for room temperature storage.
Supplied with loading dye for sample DNA. |