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Description
pBR322 DNA/AluI Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose gels. pBR322 DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
pBR322 DNA/AluI Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading onto agarose gels.
Features
Sizing and approximate quantification of small DNA fragments.
Sharp bands.
Premixed with 6X DNA Loading Dye for direct loading and room temperature storage.
Supplied with loading dye for sample DNA.
Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C. |