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Description
Lambda DNA/HindIII Marker, ready-to-use, is recommended for sizing of linear double-stranded large DNA on agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
Lambda DNA/HindIII Marker, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading (after heating) onto agarose gels.
Features
Sizing and approximate quantification of large DNA fragments.
Sharp bands.
Premixed with 6X DNA Loading Dye for a direct loading (after heating) and for room temperature storage.
Supplied with loading dye for sample DNA.
Storage and Loading Buffer (for ready-to-use markers)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C. |