|
Description
Plasmid pUC57, 2710 bp in length, is a derivative of pUC19. pUC57 MCS contains 6 restriction sites with protruding 3’-ends, that are resistant to E.coli exonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact positions of the genetic elements are shown on the map (termination codons included). DNA replication initiates at position 890 (+/- 1) and proceeds in the direction indicated. The bla gene nucleotides 2510-2442 (compl. strand) code for a signal peptide.
Additional Information
•CAP protein binding site – 615-578 (compl. strand);
•mRNA (LacZ) starts at nt position 531 (compl. strand);
•lac repressor binding site – 531-511 (compl. strand).
GenBank/EMBL Accession Number
Y14837.
Features
Purified by chromatography using proprietary patented technology.
More than 90% in the supercoiled form.
Isolated from E.coli (dam+, dcm+).
For DNA sequence, sequence analysis and map creation see free on-line tool REviewer.
Applications
Cloning.
Sequencing of insert DNA.
Generation of nested deletions with ExoIII.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |